General fluorescence
The goal the tutorials is to provide introductory information for the analysis and interpretation of fluorescence data acquired by time-tagged time resolved (TTTR) data. The analysis of TTTR-data includes:
- Fluorescence decay analysis
- Fluorescence correlation spectroscopy
- Single molecule detection
The software ChiSurf implements the methods for data analysis and interpretation presented by the tutorials. ChiSurf is developed in Python for 64bit-Windows machines and provided as a downloadable installer.
Fluorescence decay analysis
- Global analysis of multiple fluorescence decays
- Generation of fluorescence decay histograms based on TTTR data
- Analysis of time-resolved anisotropy decays
- Analysis of FRET quenched fluorescence decays by physical model functions 1
Fluorescence correlation spectroscopy
- Analysis of FCS curves
- Correlation of TTTR-data by efficient correlation algorithms 2